human wnt2 protein  (Novus Biologicals)

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Long non-coding RNA LINC00968 attenuates drug resistance of breast cancer cells through inhibiting the Wnt2/β-catenin signaling pathway by regulating WNT2

doi: 10.1186/s13046-019-1100-8

Figure Lengend Snippet: Primer sequences for RT-qPCR

Article Snippet: The detection of WNT2 in cell culture medium was performed according to the manufacture’s protocols provided by Human Protein WNT2 ELISA kit (CSB-EL026133HU, Sino-American Biotechnology Co., Ltd., Hunan, Hubei, China).

Techniques: Sequencing

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Long non-coding RNA LINC00968 attenuates drug resistance of breast cancer cells through inhibiting the Wnt2/β-catenin signaling pathway by regulating WNT2

doi: 10.1186/s13046-019-1100-8

Figure Lengend Snippet: The potential involvement of the LINC00968/HEY1/WNT2 axis mediated Wnt2/β-catenin signaling pathway in breast cancer. a selection thermogram of GEO dataset GSE26910. The horizontal coordinate represents the samples, and the vertical coordinate represents the genes. The upper right histogram is the color gradation. Each rectangle corresponds to one gene expression in one sample. Each column signifies gene expression of each sample; dendrogram on the left side represents cluster analysis of different genes from different samples; uppermost crossband represents sample type and upper right pane signifies color reference, while blue is the normal control sample and red is tumor sample. b the expression pattern of LINC00968 in cancer tissues and normal tissues of various cancers according to TCGA database. c the expression of LINC00968 in breast cancer tissues and adjacent normal tissues. d the survival analysis of LINC00968 expression and survival rate of patients. e the subcellular localization of LINC00968 in breast cancer cells as detected by FISH assay. f the binding status of LINC00968 to HEY1 as confirmed by RIP assay. g the binding sites of HEY1 to the WNT2 promoter as predicted by online Jaspar website ( http://jaspar.genereg.net/ ). h the binding status of HEY1 to the WNT2 promoter as verified by ChIP assay. i the interaction between LINC00968 and WNT2 as verified by Dual luciferase reporter gene assay. * p < 0.05 vs. the NC group. j the mRNA expression of INC00968 and WNT2 as determined by RT-qPCR. k the protein levels of INC00968 and WNT2 as evaluated by Western blot analysis. l the expression of WNT2 in cell culture medium as assayed by ELISA. * p < 0.05 vs. the control group; # p < 0.05 vs. the blank and NC groups; & p < 0.05 vs. the LINC00968 vector group. m the mRNA expression of LINC00968, WNT2, and β-catenin in breast cancer tissues and normal adjacent tissues as measured by RT-qPCR. n the protein level of WNT2 and the phosphorylation levels of β-catenin and GSK3β. o the level of LINC00968 determined by Northern blot analysis. * p < 0.05 vs. the adjacent normal tissues

Article Snippet: The detection of WNT2 in cell culture medium was performed according to the manufacture’s protocols provided by Human Protein WNT2 ELISA kit (CSB-EL026133HU, Sino-American Biotechnology Co., Ltd., Hunan, Hubei, China).

Techniques: Selection, Gene Expression, Control, Expressing, Binding Assay, Luciferase, Reporter Gene Assay, Quantitative RT-PCR, Western Blot, Cell Culture, Enzyme-linked Immunosorbent Assay, Plasmid Preparation, Phospho-proteomics, Northern Blot

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Long non-coding RNA LINC00968 attenuates drug resistance of breast cancer cells through inhibiting the Wnt2/β-catenin signaling pathway by regulating WNT2

doi: 10.1186/s13046-019-1100-8

Figure Lengend Snippet: KEGG pathway enrichment analysis of the target genes of LINC00968

Article Snippet: The detection of WNT2 in cell culture medium was performed according to the manufacture’s protocols provided by Human Protein WNT2 ELISA kit (CSB-EL026133HU, Sino-American Biotechnology Co., Ltd., Hunan, Hubei, China).

Techniques:

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Long non-coding RNA LINC00968 attenuates drug resistance of breast cancer cells through inhibiting the Wnt2/β-catenin signaling pathway by regulating WNT2

doi: 10.1186/s13046-019-1100-8

Figure Lengend Snippet: LINC00968 contributes to inhibition of the Wnt2/β-catenin signaling pathway, elevated E-cadherin level and downregulated Vimentin level. a the mRNA expression of β-catenin, GSK3β and Vimentin in MCF-7/ADM cells after plasmid delivery in each group as measured by RT-qPCR. b protein bands of β-catenin/p-β-catenin, GSK3β/p-GSK3β and Vimentin/p-Vimentin in MCF-7/ADM cells after plasmid delivery of each group as determined by Western blot analysis. c histogram of levels of β-catenin/p-β-catenin, GSK3β/p-GSK3β and Vimentin/p-Vimentin in each group. d protein bands of LINC00968 in MCF-7/ADM cells after plasmid delivery of each group. e LINC00968 protein level in each group detected by Northern blot analysis. f the mRNA expression of β-catenin, GSK3β and Vimentin in KPL-4/ADM cells after plasmid delivery in each group as measured by RT-qPCR. g protein bands of β-catenin/p-β-catenin, GSK3β/p-GSK3β and Vimentin/p-Vimentin in KPL-4/ADM cells after plasmid delivery of each group as determined by Western blot analysis. h histogram of levels of β-catenin/p-β-catenin, GSK3β/p-GSK3β and Vimentin/p-Vimentin in each group. i protein bands of LINC00968 in KPL-4/ADM cells after plasmid delivery of each group as evaluated by Northern blot analysis. j histogram of LINC00968 protein level in each group. * p < 0.05 vs. the control group; # p < 0.05 vs. the blank and NC groups; & p < 0.05 vs. the LINC00968 vector + WNT2 vector group

Article Snippet: The detection of WNT2 in cell culture medium was performed according to the manufacture’s protocols provided by Human Protein WNT2 ELISA kit (CSB-EL026133HU, Sino-American Biotechnology Co., Ltd., Hunan, Hubei, China).

Techniques: Inhibition, Expressing, Plasmid Preparation, Quantitative RT-PCR, Western Blot, Northern Blot, Control

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Long non-coding RNA LINC00968 attenuates drug resistance of breast cancer cells through inhibiting the Wnt2/β-catenin signaling pathway by regulating WNT2

doi: 10.1186/s13046-019-1100-8

Figure Lengend Snippet: Overexpressed LINC00968 or silenced WNT2 contributes to reduced drug resistance of breast cancer cells. a drug resistance of transfected MCF-7/ADM cells to ADR as evaluated by CCK-8 assay. b drug resistance of transfected MCF-7/ADM cells to Taxel as determined by CCK-8 assay. c drug resistance of transfected MCF-7/ADM cells to VCR as assessed by CCK-8 assay. d protein bands of MRP1, BCRP, and P-gp in transfected MCF-7/ADM cells ad determined by Western blot analysis. e histogram of MRP1, BCRP, and P-gp protein levels in each group. f drug resistance of transfected KPL-4/ADM cells to ADR as evaluated by CCK-8 assay. g drug resistance of transfected KPL-4/ADM cells to Taxel as determined by CCK-8 assay. h drug resistance of transfected KPL-4/ADM cells to VCR as assessed by CCK-8 assay. i protein bands of MRP1, BCRP, and P-gp in transfected KPL-4/ADM cells ad determined by Western blot analysis. j histogram of MRP1, BCRP, and P-gp protein levels in each group. * p < 0.05 vs. the control group; # p < 0.05 vs. the blank and NC groups; & p < 0.05 vs. the LINC00968 vector + WNT2 vector group

Article Snippet: The detection of WNT2 in cell culture medium was performed according to the manufacture’s protocols provided by Human Protein WNT2 ELISA kit (CSB-EL026133HU, Sino-American Biotechnology Co., Ltd., Hunan, Hubei, China).

Techniques: Transfection, CCK-8 Assay, Western Blot, Control, Plasmid Preparation

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Long non-coding RNA LINC00968 attenuates drug resistance of breast cancer cells through inhibiting the Wnt2/β-catenin signaling pathway by regulating WNT2

doi: 10.1186/s13046-019-1100-8

Figure Lengend Snippet: Overexpressed LINC00968 or silenced WNT2 decrease colony formation ability of breast cancer cells. a the colony formation ability of transfected MCF-7 cells in each group as assessed by clonogenic assay. b histogram of the number of forming colony units in each group. c the colony formation ability of transfected KPL-4 cells in each group as evaluated by clonogenic assay. d histogram of the number of forming colony units in each group. * p < 0.05 vs. the control group; # p < 0.05 vs. the blank and NC groups; & p < 0.05 vs. the LINC00968 vector + WNT2 vector group

Article Snippet: The detection of WNT2 in cell culture medium was performed according to the manufacture’s protocols provided by Human Protein WNT2 ELISA kit (CSB-EL026133HU, Sino-American Biotechnology Co., Ltd., Hunan, Hubei, China).

Techniques: Transfection, Clonogenic Assay, Control, Plasmid Preparation

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Long non-coding RNA LINC00968 attenuates drug resistance of breast cancer cells through inhibiting the Wnt2/β-catenin signaling pathway by regulating WNT2

doi: 10.1186/s13046-019-1100-8

Figure Lengend Snippet: Overexpressed LINC00968 or silenced WNT2 decreases migration and invasion abilities of breast cancer cells. a the migration ability of MCF-7 cells after plasmid delivery in each group as evaluated by the scratch test. b the invasion ability of MCF-7 and MCF-7/ADM cells after plasmid delivery in each group as determined by the Transwell assay. c the cell migration rate in each group. d the number of invasive cells in each group. * p < 0.05 vs. the control group; # p < 0.05 vs. the blank and NC groups; & p < 0.05 vs. the LINC00968 vector + WNT2 vector group

Article Snippet: The detection of WNT2 in cell culture medium was performed according to the manufacture’s protocols provided by Human Protein WNT2 ELISA kit (CSB-EL026133HU, Sino-American Biotechnology Co., Ltd., Hunan, Hubei, China).

Techniques: Migration, Plasmid Preparation, Transwell Assay, Control

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Long non-coding RNA LINC00968 attenuates drug resistance of breast cancer cells through inhibiting the Wnt2/β-catenin signaling pathway by regulating WNT2

doi: 10.1186/s13046-019-1100-8

Figure Lengend Snippet: Overexpressed LINC00968 or silenced WNT2 contributes to promoted apoptosis of breast cancer cells. a the apoptosis of MCF-7 cells after transduction in each group as analyzed by flow cytometry. b the cell apoptosis rate in each group. c protein bands of Bax, cleaved-PARP, cleaved-caspase3 and Bcl-2 in MCF-7/ADM cells after transduction in each group as measured by Western blot analysis. d the protein levels of Bax, cleaved-PARP, cleaved-caspase3 and Bcl-2 in each group. * p < 0.05 vs. the control group; # p < 0.05 vs. the blank and NC groups; & p < 0.05 vs. the LINC00968 vector + WNT2 vector group

Article Snippet: The detection of WNT2 in cell culture medium was performed according to the manufacture’s protocols provided by Human Protein WNT2 ELISA kit (CSB-EL026133HU, Sino-American Biotechnology Co., Ltd., Hunan, Hubei, China).

Techniques: Transduction, Flow Cytometry, Western Blot, Control, Plasmid Preparation

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Long non-coding RNA LINC00968 attenuates drug resistance of breast cancer cells through inhibiting the Wnt2/β-catenin signaling pathway by regulating WNT2

doi: 10.1186/s13046-019-1100-8

Figure Lengend Snippet: Overexpressed LINC00968 or silenced WNT2 restrains transplantation tumor growth in nude mice. a the tumor volume in nude mice of each group b the tumor weight in nude mice of each group. * p < 0.05 vs. the control group; # p < 0.05 vs. the blank and NC groups; & p < 0.05 vs. the LINC00968 vector + WNT2 vector group

Article Snippet: The detection of WNT2 in cell culture medium was performed according to the manufacture’s protocols provided by Human Protein WNT2 ELISA kit (CSB-EL026133HU, Sino-American Biotechnology Co., Ltd., Hunan, Hubei, China).

Techniques: Transplantation Assay, Control, Plasmid Preparation

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Long non-coding RNA LINC00968 attenuates drug resistance of breast cancer cells through inhibiting the Wnt2/β-catenin signaling pathway by regulating WNT2

doi: 10.1186/s13046-019-1100-8

Figure Lengend Snippet: LINC00968 was lowly expressed, whereas WNT2 was highly expressed in breast cancer tissues and cells. LINC00968 targeted and negatively regulated WNT2 via the transcriptional repressor HEY1. Overexpressed LINC00968 reduced drug resistance, migration, invasion and epithelial-mesenchymal transition of breast cancer cells through inhibiting the activation of the Wnt2/β-catenin signaling pathway via suppression of WNT2

Article Snippet: The detection of WNT2 in cell culture medium was performed according to the manufacture’s protocols provided by Human Protein WNT2 ELISA kit (CSB-EL026133HU, Sino-American Biotechnology Co., Ltd., Hunan, Hubei, China).

Techniques: Migration, Activation Assay

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Long non-coding RNA LINC00968 attenuates drug resistance of breast cancer cells through inhibiting the Wnt2/β-catenin signaling pathway by regulating WNT2

doi: 10.1186/s13046-019-1100-8

Figure Lengend Snippet: IC 50 levels of ADR, Taxel and VCR is reduced in MCF-7/ADM and KPL-4/ADM cells after plasmid delivery

Article Snippet: The detection of WNT2 in cell culture medium was performed according to the manufacture’s protocols provided by Human Protein WNT2 ELISA kit (CSB-EL026133HU, Sino-American Biotechnology Co., Ltd., Hunan, Hubei, China).

Techniques: Plasmid Preparation, Control